What is the maximum magnification of a dissecting microscope?
What is the maximum magnification of a dissecting microscope?
100x
What is a limitation of using electron microscopes to view specimens?
What is a limitation of using electron microscopes to view specimens? You cannot view transparent specimens because of the microscope’s low resolution. Electron beams are dangerous and can only be used sparingly. You cannot view live specimens because the necessary preparation kills cells.
What is the range of a dissecting microscope?
10x – 40x
What advantages does the dissecting microscope have over the compound microscope?
The dissecting microscope is configured to allow low magnification of three-dimensional objects- objects larger or thicker than the compound microscope can accommodate. Furthermore, the two separate lenses of the binocular dissecting microscope allow one to see objects in three dimensions, i.e., in stereo.
Which part of the dissecting microscope is the most important?
objectives
What happens if you try to use coarse adjustment when the 10X lens is in place?
What happens if you try to use the coarse adjustment when the 10X lens is in place? The focus of the specimen would be unclear with a high objective power and the stage lifted high due to the coarse adjustment. There is a warning it is not good to change coarse focus under high power.
When using a microscope How will we see a specimen?
Look through the eyepieces (4) and move the focus knob (1) until the image comes into focus. Adjust the distance between the eyepieces (4) until you can see the sample clearly with both eyes simultaneously (you should see the sample in 3D).
How can you avoid getting air bubbles on your wet mount?
Place a sample on the slide. Using a pipette, place a drop of water on the specimen. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent. This method will help prevent air bubbles from being trapped under the cover slip.
What are the advantages and disadvantages of using a hanging drop mount?
Advantages: Like the wet mount, the hanging drop method preserves cell shape and arrangement. The Vaseline-sealed depression also slows down the drying-out process, so the organisms can be observed for longer periods. Disadvantages: The hanging drop method is also far too risky to use with highly pathogenic organisms.
Why is the hanging drop method used for observing motility?
In wet mount, though the shape and size of bacteria can be observed, motility may be hampered, as the suspension is pressed between the slide and the cover slip. That is why; hanging drop preparation or motility test is performed for clear observation of the motility of bacteria, besides their shape and size.
Why is petroleum jelly used in the hanging drop procedure?
In this method, a drop of culture is placed on a coverslip that is encircled with petroleum jelly (or any other sticky material). The coverslip and drop are then inverted over the well of a depression slide. The drop hangs from the coverslip, and the petroleum jelly forms a seal that prevents evaporation.