What is the mobile phase and stationary phase in gas chromatography?

What is the mobile phase and stationary phase in gas chromatography?

In gas chromatography (GC), the mobile phase is an inert gas such as helium. The mobile phase carries the sample mixture through what is referred to as a stationary phase. The stationary phase is a usually chemical that can selectively attract components in a sample mixture.

Is the stationary phase in gas chromatography polar?

The polyethyleneglycol (PEG) stationary phases are the most frequently used polar sorbents. PEG 400 is a suitable sorbent for the separation of alcohols, ethers, aldehydes and other compounds with low boiling points, while Carbowax 20M can be used for the separation of polar compounds with higher boiling points.

What does the stationary phase do in chromatography?

The stationary phase remains fixed in place while the mobile phase carries the components of the mixture through the medium being used. The stationary phase acts as a constraint on many of the components in a mixture, slowing them down to move slower than the mobile phase.

Which factor is not affect in stationary phases in paper chromatography?

Paper Chromatography :- Stationary phase has a bound or abosorbed water present with the cellulose of the chromatographic paper and the mobile phase is an organic solvent which is immiscible with stationary phase.

Is a technique in which components of a mixture are separated by distributing them between two phases a stationary phase and a mobile phase?

Chromatography is defined by IUPAC as a ‘physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary (stationary phase) while the other (the mobile phase) moves in a definite direction’ (MacNaught and Wilkinson, 1997).

How does the solvent work in chromatography?

The solvent, which is in the bottom of the container, travels up the layer of adsorbent by capillary action, passes over the spot and, as it continues up, moves the compounds in the mixture up the plate at different rates resulting in separation of the compounds.